Isolation of Bacterial Pathogens from Clinical Cases of Mastitis in Buffaloes

A total of 60 milk samples, from buffaloes showing clinical and recurrent type of mastitis, as confirmed by both California mastitis test and Wide side test, were collected for bacteriological examination. As manh as 65 bacterial pathogens were isolated, using different selective media and their biochemical characterization revealed Staphylococcus aureus (20%), Streptococcus spp. (18.5%), Escherichia coli (4.6%), Pseudomonas aeuroginosa (3%), Salmonella spp. (7.6%), Klebsiella pneumonia (1.5%), Proteus vulgaris (1.5%), Actinobacillus spp. (1.5%), Actinomyces (1.5%), Corynebacterium spp. (1.5%), Bacillus spp. (3%), Nocardia (1.5%), Haemophilus (3%), and other unidentified gram negative organisms (30%). Among the contagious pathogens, the prevalence of Staphylococcus and Streptococcus was almost equal whereas Escherichia coli and Salmonella were predominant among environmental pathogens.